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KMID : 0616120180490010015
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Korean Journal of Pharmacognosy
2018 Volume.49 No. 1 p.15 ~ p.22
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Simultaneous Analysis of Phenolic Compounds in Geranium thunbergii Using UPLC
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Kim Se-Gun
Ramakanta Lamichhane
Lee Kyung-Hee
Pandeya Prakash Raj
Shim Sang-Yeon
Jung Hyun-Ju
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Abstract
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The aim of this study was to develop a UPLC method for simultaneous analysis of 8 phenolic compounds including gallic aicd (1), protocatechuic acid (2), methyl gallate (3), ellagic acid (4), kaempferol-3-arabinofranosyl-7-rhamnoside (5), kaempferitrin (6), afzelin (7) and kaempferol-7-rhamnoside (8) isolated from Geranium thunbergii which has been traditionally used as anti-diarrheal agent. The UPLC method was optimized and validated using Halo C18 column (4.6×100 mm, 2.7 μm) consisting of MeOH and 0.1% formic acid at 260 nm in 25 minutes. In quantitative analysis of 8 compounds in MeOH extract of G. thunbergii, contents of 4-6 were 12.39, 20.52 and 21.45 mg/g, respectively. These compounds were measured as major phenolic compounds in G. thunbergii and can be useful as marker compounds for its quality control. These results suggest that the UPLC method can be contributed as basic data for quality evaluation of herbal preparations.
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KEYWORD
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Geranium thunbergii , UPLC , Phenolic compounds , Method validation ,
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